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KMID : 1059519890330030287
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Journal of the Korean Chemical Society
1989 Volume.33 No. 3 p.287 ~ p.294
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Study of the Separation and Elution Behavior of Phenols as Priority Pollutants in Reversed Phase Liquid Chromatography
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Lee Dai-Woon
Lee Sun-Kyung
Yook Keun-Sung
Lee Won
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Abstract
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The optimum condition for the separation of priority pollutant phenols using isocratic elution has been determined. The elution behavior of eleven phenols has been also studied to interpret the retention. The reversed phase liquid chromatographic methods were performed on a μ-Bondapak C18 column with methanol-water, acetonitrile-water, and THF water mixtures as mobile phases. The COF method, where Snyder's solvent triangle concept was combined with a mixture-design statistical technique, was used to optimize the strength and selectivity of solvents for the separation of phenols. The optimum solvent composition, which gives a complete separation of eleven phenols, was found to be MeOH:ACN:H2O = 7:40:53. The plots of ln k' vs. -ΔHo and ∑π of phenols showed relatively good linearities. Effect of van der Waals volume, pi-energy and hydrogen bonding on the retention of phenols were investigated. The following equation with the correlation coefficient of 0.9927 for ACN-water solvent system was obtained; log k' = 2.515 × 10-2 VWV - 1.301 × 10-1 E - 3.674 × 10-1.
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